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MICRO 130
General Microbiology (3) AA/NS1 and AS/NS
3 hours lecture
per week
Recommended Preparation: MATH 25 and CHEM 100 or higher
level chemistry or biochemistry course
The
fundamentals of microbiology with an emphasis on microorganisms
as they affect people, property and the environment.
Broad aspects of biochemistry, genetics, molecular biology,
physiology, host-parasite relationships, infectious diseases, immunology,
public health, epidemiology, food microbiology, and environmental
microbiology will be covered.
Upon successful
completion of MICRO 130, the student should be able to:
Describe the organization of life at the cellular and subcellular
levels.
Describe the main characteristics of bacteria, including their
morphology, growth, reproduction and classification.
Understand and describe in general terms, the fundamental biochemistry
of bacterial metabolism and compare it to eukaryotic cell metabolism.
Understand and describe the basic principles of molecular genetics
as they relate to cell division, mutation, genetic engineering,
bacterial virulence and antibiotic resistance.
Understand and describe the fundamental principles of the host-parasite
relationship both in health and disease.
Describe the components of the human immune system and understand
how these components interact in generating an immune response.
Mathematically express the growth characteristics of a bacterial
culture.
Describe the major and the common infectious diseases of humans.
Understand and apply methods of microbial control that can be
used to prevent both the transmission of infectious diseases and
the spoilage of foods and textiles.
Read and understand microbiology articles in nursing journals
and the popular press.
MICRO 135
Microbiology for the Health Professions (3) AA/NS1* and AS/NS*
3 hours lecture
per week
Prerequisite(s): Credit or concurrent
BIOL 130 or ZOOL 141
Recommended Preparation: CHEM 151 or 161
This
course is an introduction to the study of microorganisms, host-parasite
relationships, control and characterization of disease-causing organisms
with emphasis on health applications.
Upon successful
completion of MICRO 135, the student should be able to:
Describe morphology, growth and metabolism in bacteria.
Identify principles of host-parasite relationships.
Describe principles and methods of control of microorganisms.
Describe the principles and actions of antibiotics.
Describe specimen collection and handling.
Describe the structure and chemistry of immunoglobulins.
Discuss the mechanisms that protect the body from disease and/or
injury.
MICRO 140
General Microbiology Laboratory (2) AA/NS1
4 hours lecture/lab
per week
Prerequisite(s): Credit or concurrent
enrollment in MICRO 130 or 135
Recommended Preparation: MATH 25 and CHEM 100 or higher
level chemistry or biochemistry
Fundamental
laboratory aspects of microbiology with a public health and medical
emphasis.
Upon successful
completion of MICRO 140, the student should be able to:
Use the metric system and scientific notation.
Use and properly care for the compound microscope, including
the oil immersion lens.
Prepare, examine and accurately interpret various stained slide
specimens including gram stained, capsule stained, endospore
stained and flagella stained specimens.
Understand and properly execute the aseptic transfer of bacterial
cultures.
Use sterile pipettes aseptically and accurately.
Demonstrate the ubiquity of microbes as part of our normal flora
and as present in the environment.
Understand and demonstrate the principles and the techniques
which are used to control microorganisms such as antibiotics, food
preservatives, and the chemical, physical disinfecting and sterilizing
agents.
Enumerate the bacteria in food and water samples and mathematically
predict the growth characteristics of these bacteria.
Understand and demonstrate the effect of different personal hygiene
practices on our normal flora and on pathogens.
Demonstrate and understand the various nutritional requirements
and characteristics of medically important bacteria.
Demonstrate the ability to isolate and to identify common human
commensal bacteria in pure culture.
Demonstrated the principles involved in the transmission of pathogenic
organisms by the common routes.
Demonstrate the thinking skills needed to critically observe,
measure, evaluate and interpret experimental data and the creativity
needed to formulate hypotheses to explain the data.
MICRO 230
Molecular Biology (3)
6 hours lecture/lab
per week
Prerequisite(s): MICRO 130 or MICRO 135
or BIOL 171; MICRO 140 or MLT 107 or BIOL 171L; CHEM 151 or higher;
CHEM 151L or higher
This course
covers the fundamental theoretical and laboratory aspects of molecular
biology. The basic principles which govern the
structure and function of proteins, nucleic acids and macromolecular
complexes will be studied. Students will learn and become
proficient at performing the fundamental laboratory procedures of
biotechnology.
Upon successful
completion of MICRO 230, the student should be able to:
Describe the structure of proteins, nucleic acids and macromolecular
complexes.
Describe the function of nucleic acids, proteins and macromolecules
in DNA replication, transcription, translation, mutagenesis and
DNA repair.
Describe the regulation of gene activity in prokaryotes and eukaryotes.
Describe basic principles and techniques of molecular biology
including the use of plasmids and transposons
to generate recombinant DNA.
Prepare, sterilize and dispense the basic types of media used
for the cultivation of bacteria.
Operate all the basic equipment of a molecular biology laboratory,
including but not limited to large autoclaves and bench top autoclaves,
water distillation apparatus, biological safety cabinets, spectrophotometers
and ELISA readers, electrophoresis equipment, centrifuges and microcentrifuges.
Perform agarose gel electrophoresis.
Isolate and quantitate chromosomal and plasmid DNA from bacteria.
Perform and analyze restriction enzyme digestions of DNA.
Prepare and screen a genomic library.
Prepare enzyme labeled probes and perform southern blots.
Perform polymerase chain reactions under a variety of conditions.
Analyze DNA and amino acid sequence data by searching sequence
data bases.
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